Influence of complete denture adhesives on microbial adhesion and biofilm formation
Paula Łasica1, Dr. Urszula Wnorowska2, Prof. Teresa Sierpińska1, Prof. Robert Bucki2
1 Medical University of Białystok, Department of Prosthetic Dentistry,
2 Medical University of Białystok, Department of Medical Microbiology and Nanobiomedical Engineering
Objectives
This study aimed to ascertain whether various types of complete denture adhesives have an impact on the attachment and growth of Candida albicans and Streptococcus mutans in single- and mixed-species settings.
Materials and Methods
The quantity of commercially available cream adhesive products (n=6) was applied on sterile coverslips within the vertical laminar flow chamber. 1x106 CFU of C. albicans and S. mutans (in single- and mixed-species settings) was applied directly onto the creme adhesive-coated surfaces. After incubating coverslips at 37°C for 90 minutes, the specimens were cleansed gently with PBS to eliminate unbound cells. The adhered cells were detached by sonicating the coverslips for 10 minutes. Diluting of microbial suspension in a serial manner from 10−1 to 10−6 was performed and the drop technique was utilized to quantify the microbial adhesion to the denture-coated surfaces. In another experimental setting, the culture medium was switched out following the 90-minute incubation time and the specimens were left to incubate for a further 24 hours to form the biofilm. Biofilm formation was evaluated through resazurin-based staining assay.
Results
Overall, in mixed-species settings of C. albicans and S. mutans, it was found that fewer microorganisms adhered to the cream adhesives compared to when each species was applied individually. The results after 24 hours of incubation were similar. In effect, biofilm formation was also lower in mixed-species settings compared to single-species settings of C. albicans or S. mutans. Furthermore, the analysis revealed notable variations in the quantity of adhered cells depending on the presence of PBS or saliva in the environment surrounding the adhesive-coated specimens. Specifically, it was observed a higher quantity of fungi and bacteria adhering in the presence of PBS compared to a saliva environment (50%/50%).
Conclusions
These findings underscore the importance of considering both microbial interactions and environmental factors in understanding the efficacy of denture adhesives in preventing microbial adhesion and biofilm formation.